RESUMO
BACKGROUND: Stenotrophomonas maltophilia is an emerging opportunistic pathogen. The increasing incidence is of particular concern in patients with cystic fibrosis (CF). Since 2012, the Western France has witnessed high annual prevalence of S. maltophilia colonization/infection. This retrospective cohort study investigated the epidemiology of S. maltophilia emergence in the CF center of Roscoff, Western France, a region of high prevalence of CF in Europe. METHODS: All CF patients with S. maltophilia isolated in respiratory samples between December 2013 and February 2017 were included. For each patient the colonization status with S. maltophilia was determined. The epidemiological and microbiological characteristics collected were compared between colonization statuses. RESULTS: S. maltophilia was isolated in 90 patients (42 males, 48 females). Mean age at first colonization was 24.4±13.5 years. Annual prevalence since 2013 was high (16-17.9%), but stable. This high prevalence is mainly due to a high rate of intermittent colonization. Only 2.8% of CF patients showed chronic colonization, with significantly more frequent co-colonization by methicillin-susceptible Staphylococcus aureus (P<0.0001) and Pseudomonas aeruginosa (P<0.05). During chronic colonization, S. maltophilia acquired resistance to cotrimoxazole and ß-lactams. Interestingly, there were cases of decolonization. CONCLUSION: This is the first epidemiological report of S. maltophilia in a French CF center. Prevalence was stable but above the national average. Most cases were intermittent; chronic colonization was rare.
Assuntos
Fibrose Cística/epidemiologia , Infecções por Bactérias Gram-Negativas/epidemiologia , Stenotrophomonas maltophilia , Adolescente , Adulto , Criança , Estudos de Coortes , Fibrose Cística/complicações , Fibrose Cística/microbiologia , Feminino , França/epidemiologia , Infecções por Bactérias Gram-Negativas/complicações , Humanos , Masculino , Prevalência , Infecções Respiratórias/complicações , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Estudos Retrospectivos , Stenotrophomonas maltophilia/isolamento & purificação , Adulto JovemAssuntos
Infecções por Helicobacter/diagnóstico , Helicobacter/isolamento & purificação , Doença de Raynaud/microbiologia , Adulto , Antibacterianos/uso terapêutico , Feminino , Helicobacter/efeitos dos fármacos , Infecções por Helicobacter/tratamento farmacológico , Humanos , Imunocompetência , Doença de Raynaud/complicações , Resultado do TratamentoAssuntos
Capnocytophaga , Infecções por Bactérias Gram-Negativas , Meningites Bacterianas/microbiologia , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/terapia , Humanos , Masculino , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/terapia , Pessoa de Meia-IdadeRESUMO
PURPOSE: Bartonella is an increasingly isolated emerging pathogen that can cause severe illness in humans, including cat scratch disease (CSD). The bacteria are difficult to grow and thus many detection methods have been developed, especially molecular. We previously developed a PCR method targeting ribC to identify Bartonella sp. A manufactured kit (RealCycler BART, Progenie Molecular) was commercialised shortly thereafter for the detection of Bartonella infection, including Bartonella henselae. METHODOLOGY: We performed a comparison between this test and our in-house PCR assay on 73 lymphadenopathy samples sent to the laboratory for suspicion of CSD.Results/Key findings. Among the 28 positive samples for Bartonella, 21 were identified by the two PCR assays, and seven by the commercial kit only. CONCLUSION: The performance of this commercial kit suggests that it could be a suitable alternative to our in-house PCR assay, highlighting the importance of the molecular methods used to diagnose CSD.
